Category: cDNA

Polypeptide components and binding functions of neuron-glia cell adhesion molecules.

Neuron-glia cell adhesion molecule (Ng-CAM) has beforehand been proven to be current completely on neurons and to mediate adhesion between neuronal membranes and glial cells. Within the current examine, its chain construction, binding capabilities, and relation to N-CAM (the opposite recognized CAM on neurons) had been investigated additional. Three polypeptide parts of rooster Ng-CAM (Mr 200,000, 135,000,

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Expression of monocarboxylate transporter MCT1 by brain endothelium and glia in adult and suckling rats.

A polyclonal affinity-purified antibody to the carboxyl-terminal finish of the rat monocarboxylate transporter 1 (MCT1) was generated in chickens and utilized in immunocytochemical research of mind tissue sections from grownup and suckling rats. The antibody recognized a 48-kDa band on immunoblots and stained tissue sections of coronary heart, cecum, kidney, and skeletal muscle, in step with the reported molecular mass and mobile expression

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Topoisomerase II is a structural component of mitotic chromosome scaffolds.

We’ve got obtained a polyclonal antibody that acknowledges a serious polypeptide element of hen mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr 170,000. Oblique immunofluorescence and subcellular fractionation experiments affirm that it’s current in each mitotic chromosomes and interphase nuclei. Two strains of proof counsel that this protein is DNA topoisomerase II, an plentiful nuclear enzyme that

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